Anticorps Recombinant de lapin anti-CD206

CD206 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G15

N° de cat : 81525-1-PBS

Synonymes

1G15, CLEC13D, CLEC13DL, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like

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Galerie de données de validation

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  WB analysis using 81525-1-RR (same clone as 81525-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81525-1-RR (CD206 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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Informations sur le produit

81525-1-PBS cible CD206 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mannose receptor, C type 1
• Masse moléculaire calculée: 166 kDa
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: NM_002438
• Symbole du gène: CD206
• Identification du gène (NCBI): 4360
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.

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