Anticorps Monoclonal anti-CD206

CD206 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

APC Fluorescent Dye

Publications(3)

CloneNo.

15-2

N° de cat : APC-65155

Synonymes

15-2, CLEC13D, CLEC13DL, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like

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Galerie de données de validation

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  FC experiment of human PBMCs using APC-65155

  1x10^6 GM-CSF treated human PBMCs were surface stained with 5 ul APC Anti-Human CD206 (15-2) (APC-65155, Clone:15-2) (red) or APC Mouse IgG1 Isotype Control (MOPC-21) (APC-65124, Clone: MOPC-21) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul APC Anti-Human CD206 (15-2) (APC-65155, Clone:15-2) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: GM-CSF treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 3 publications below

Informations sur le produit

APC-65155 cible CD206 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Récepteur de mannose humain purifié
• Nom complet: mannose receptor, C type 1
• Masse moléculaire calculée: 166 kDa
• Numéro d’acquisition GenBank: NM_002438
• Symbole du gène: CD206
• Identification du gène (NCBI): 4360
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: L'anticorps purifié est conjugué à l'allophycocyanine (APC) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.

Publications

Species	Application	Title
	FC	Int J Mol Sci; C-X-C Motif Chemokine 3 Promotes the Inflammatory Response of Microglia after Escherichia coli-Induced Meningitis; Authors - Xinyi Qu
human	FC	Cell Mol Life Sci; Tumor-associated macrophage-derived exosomal miR21-5p promotes tumor angiogenesis by regulating YAP1/HIF-1α axis in head and neck squamous cell carcinoma; Authors - Quan Yan
human	FC	Front Immunol; Identification of a distinct cluster of GDF15high macrophages induced by in vitro differentiation exhibiting anti-inflammatory activities; Authors - Chaochao Dai

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