Anticorps Polyclonal de lapin anti-CD21

CD21 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 24374-1-AP

Synonymes

C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9

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Galerie de données de validation

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  WB analysis using 24374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24374-1-AP (CD21 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 24374-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24374-1-AP (CD21 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 24374-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24374-1-AP (CD21 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Raji,
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

24374-1-AP cible CD21 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD21 Protéine recombinante Ag18058
• Nom complet: complement component (3d/Epstein Barr virus) receptor 2
• Masse moléculaire calculée: 1092 aa, 119 kDa
• Poids moléculaire observé: 145 kDa
• Numéro d’acquisition GenBank: BC136394
• Symbole du gène: CD21
• Identification du gène (NCBI): 1380
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

Publications

Species	Application	Title
human	IF	Nat Commun; B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma.; Authors - Johannes Griss
human	IF	Clin Transl Med; Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy; Authors - Qing Wang
human	IHC，IF	Front Immunol; A Standardized Analysis of Tertiary Lymphoid Structures in Human Melanoma: Disease Progression- and Tumor Site-Associated Changes With Germinal Center Alteration.; Authors - Franziska Werner
human	IHC	Cell Death Dis; Single-cell landscape identified SERPINB9 as a key player contributing to stemness and metastasis in non-seminomas; Authors - Zhouliang Bian

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