Anticorps Recombinant de lapin anti-CD22

CD22 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230550C11

N° de cat : 83303-1-PBS

Synonymes

CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22

---

Galerie de données de validation

• 

  WB analysis using 83303-1-RR (same clone as 83303-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83303-1-RR (CD22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83303-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83303-1-RR (same clone as 83303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83303-1-RR (CD22 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83303-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83303-1-RR (same clone as 83303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83303-1-RR (CD22 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83303-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83303-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83303-1-RR against Human CD22 were performed. The affinity constant is 3.33 nM.

---

Informations sur le produit

83303-1-PBS cible CD22 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD22 molecule
• Masse moléculaire calculée: 95kd
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: NM_001771.4
• Symbole du gène: CD22
• Identification du gène (NCBI): 933
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

{{ptg:RelatedPrimaryAntibodies}}