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Anticorps Recombinant de lapin anti-CD22

CD22 Recombinant Antibody for IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250078E8

N° de cat : 86128-2-PBS

Synonymes

B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule, Lyb-8, Sialic acid-binding Ig-like lectin 2

Galerie de données de validation

Filter:
  • Immunofluorescence (IF) / fluorescent staining of A20 cells using CD22 Recombinant antibody (86128-2-RR)

    IF Staining of A20 using 86128-2-RR (same clone as 86128-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A20 cells using CD22 antibody (86128-2-RR, Clone: 250078E8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86128-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86128-2-RR

    Affinity and Kinetic Characterization of 86128-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86128-2-RR against Human CD22 were performed. The affinity constant is 0.63 nM.

Informations sur le produit

86128-2-PBS cible CD22 dans les applications de IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène CD22 Protéine recombinante Eg1324
Nom complet CD22 antigen
Masse moléculaire calculée97kDa
Numéro d’acquisition GenBankNM_001043317.2
Symbole du gène Cd22
Identification du gène (NCBI) 12483
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

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