Anticorps Recombinant de lapin anti-CD22

CD22 Recombinant Antibody for IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250078E8

N° de cat : 86128-2-RR

Synonymes

B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule, Lyb-8, Sialic acid-binding Ig-like lectin 2

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Galerie de données de validation

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  IF Staining of A20 using 86128-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A20 cells using CD22 antibody (86128-2-RR, Clone: 250078E8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86128-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86128-2-RR against Human CD22 were performed. The affinity constant is 0.63 nM.

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Applications testées

• Résultats positifs en IF/ICC: A20 cells,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86128-2-RR cible CD22 dans les applications de IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD22 Protéine recombinante Eg1324
• Nom complet: CD22 antigen
• Masse moléculaire calculée: 97kDa
• Numéro d’acquisition GenBank: NM_001043317.2
• Symbole du gène: Cd22
• Identification du gène (NCBI): 12483
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

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