Anticorps Recombinant de lapin anti-CD22

CD22 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241315A1

N° de cat : 98092-1-RR

Synonymes

CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22

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Galerie de données de validation

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  FC experiment of human PBMCs using 98092-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD22 Rabbit Recombinant Antibody (98092-1-RR, Clone:241315A1) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG, Cells were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98092-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD22 Rabbit Recombinant Antibody (98092-1-RR, Clone:241315A1) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98092-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD22 Rabbit Recombinant Antibody (98092-1-RR, Clone:241315A1) and PE-conjugated Goat Anti-Rabbit IgG (left) or 0.25ug other vendor PE Anti-Human CD22 (right). Cells were co-stained with APC Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

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  Affinity and Kinetic Characterization of 98092-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98092-1-RR against Human CD22 were performed. The affinity constant is 3.11 nM.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98092-1-RR cible CD22 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD22 molecule
• Masse moléculaire calculée: 847 aa, 95 kDa
• Numéro d’acquisition GenBank: BC109306
• Symbole du gène: CD22
• Identification du gène (NCBI): 933
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

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