Anticorps Recombinant de lapin anti-CD22

CD22 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

250078D10

N° de cat : 98485-2-RR

Synonymes

B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule, Lyb-8, Sialic acid-binding Ig-like lectin 2

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98485-2-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD22 Rabbit RecAb (98485-2-RR, Clone: 250078D10) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD19 (1D3) (CL488-65290, Clone: 1D3). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98485-2-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD22 Rabbit RecAb (98485-2-RR, Clone:250078D10) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD19 (1D3) (CL488-65290, Clone: 1D3). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98485-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98485-2-RR against Mouse CD22 were performed. The affinity constant is 17.4 nM.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98485-2-RR cible CD22 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD22 Protéine recombinante Eg1324
• Nom complet: CD22 antigen
• Masse moléculaire calculée: 97kDa
• Numéro d’acquisition GenBank: NM_001043317.2
• Symbole du gène: Cd22
• Identification du gène (NCBI): 12483
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

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