Anticorps Monoclonal anti-CD22

CD22 Monoclonal Antibody for IF-P

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IF-P

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

7A2F1

N° de cat : CL594-66103

Synonymes

B cell receptor CD22, BL CAM, CD22, CD22 molecule, FLJ22814, SIGLEC 2, SIGLEC2, T cell surface antigen Leu 14

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Galerie de données de validation

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  IF Staining of human tonsillitis using CL594-66103

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66103 (CD22 antibody) at dilution of 1:100.

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  IF Staining of human tonsillitis using CL594-66103

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66103 (CD22 antibody) at dilution of 1:100.

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Applications testées

• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66103 cible CD22 dans les applications de IF-P et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD22 Protéine recombinante Ag17986
• Nom complet: CD22 molecule
• Masse moléculaire calculée: 847 aa, 95 kDa
• Numéro d’acquisition GenBank: BC109306
• Symbole du gène: CD22
• Identification du gène (NCBI): 933
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell. And this antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

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