Anticorps Monoclonal anti-LAG-3/CD223

LAG-3/CD223 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG1, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

C9B7W

N° de cat : CL488-65098

Synonymes

CD223, LAG-3, Ly66, Lag3, LAG 3

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Galerie de données de validation

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  FC experiment of BALB/C mouse splenocytes using CL488-65098

  1X10^6 unstimulated or anti-CD3/CD28 stimulated (2 days) BALB/C mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD223 (CL488-65098, Clone: C9B7W). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Anti-CD3/CD28 treated mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65098 cible LAG-3/CD223 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine de fusion CD223-Ig murine
• Nom complet: lymphocyte-activation gene 3
• Numéro d’acquisition GenBank: BC120591
• Symbole du gène: CD223
• Identification du gène (NCBI): 16768
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).

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