Anticorps Monoclonal anti-CD223

CD223 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG1, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

C9B7W

N° de cat : CL594-65098

Synonymes

CD223, LAG 3, Lag3, Ly66, lymphocyte activation gene 3

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL594-65098

  1X10^6 unstimulated or anti-CD3/CD28 stimulated (3 days) mouse splenocytes were surface co-stained with FITC Anti-Mouse CD3 and 0.5 ug CoraLite®594 Anti-Mouse CD223 (CL594-65098, Clone: C9B7W). Cells were not fixed.

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  FC experiment of mouse splenocytes using CL594-65098

  1X10^6 unstimulated (dashed line) or anti-CD3/CD28 stimulated (3 days) mouse splenocytes were surface stained with 0.5 ug CoraLite®594 Anti-Mouse CD223 (CL594-65098, Clone: C9B7W) (red), or 0.5 ug Isotype Control (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: anti-CD3/CD28 stimulated mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-65098 cible CD223 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine de fusion CD223-Ig murine
• Nom complet: lymphocyte-activation gene 3
• Numéro d’acquisition GenBank: BC120591
• Symbole du gène: Lag3
• Identification du gène (NCBI): 16768
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).