Anticorps Recombinant de lapin anti-CD226

CD226 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

11A8

N° de cat : CL488-65573

Synonymes

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

---

Galerie de données de validation

• 

  FC experiment of human PBMCs using CL488-65573

  1x10^6 human PBMCs were surface stained with 5 ul Coralite® Plus 488 CD226 Recombinant Antibody (CL488-65573, Clone:11A8) or CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4). Cells were then stained with 5 ul APC Anti-Human CD3 (OKT3) (APC-65133, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL488-65573

  1x10^6 human PBMCs were surface stained with 5 ul Coralite® Plus 488 CD226 Recombinant Antibody (CL488-65573, Clone:11A8) (right) or 5 ul CoraLite® Plus 488 Anti-Human CD226 (11A8) (CL488-65247, Clone: 11A8) (left). Cells were then stained with 5 ul APC Anti-Human CD3 (OKT3) (APC-65133, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

---

Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65573 cible CD226 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cellules NK humaines
• Nom complet: CD226 molecule
• Masse moléculaire calculée: 336 aa, 39 kDa
• Numéro d’acquisition GenBank: BC074787
• Symbole du gène: CD226
• Identification du gène (NCBI): 10666
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).

{{ptg:RelatedPrimaryAntibodies}}