Anticorps Monoclonal anti-CD23

CD23 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3H6B8

N° de cat : 60208-1-PBS

Synonymes

3H6B8, BLAST 2, BLAST-2, CD23A, CLEC4J

---

Galerie de données de validation

• 

  WB analysis using 60208-1-Ig (same clone as 60208-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60208-1-Ig (CD23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

• 

  WB analysis of DC2.4 using 60208-1-Ig (same clone as 60208-1-PBS)

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 60208-1-Ig (CD23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-1-Ig (CD23 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD23 antibody (60208-1-Ig, Clone: 3H6B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD23 antibody (60208-1-Ig, Clone: 3H6B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50220-3

  Cytometric bead array standard curve of MP50220-3, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-2-PBS. Detection antibody: 60208-1-PBS. Standard:Ag0425. Range: 0.391-100 ng/mL.

• 

  Cytometric bead array standard curve of MP50220-4

  Cytometric bead array standard curve of MP50220-4, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-1-PBS. Detection antibody: 60208-4-PBS. Standard:Ag0425. Range: 0.391-100 ng/mL.

---

Informations sur le produit

60208-1-PBS cible CD23 dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD23 Protéine recombinante Ag0425
• Nom complet: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Masse moléculaire calculée: 321 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC064417
• Symbole du gène: CD23
• Identification du gène (NCBI): 2208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.

{{ptg:RelatedPrimaryAntibodies}}