Anticorps Recombinant de lapin anti-CD23

CD23 Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241385C9

N° de cat : 84212-3-PBS

Synonymes

241385C9, Fc-epsilon-RII, Fcer2, Fcer2a, Low affinity immunoglobulin epsilon Fc receptor

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Galerie de données de validation

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  WB analysis of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01134-2

  Sandwich ELISA standard curve of MP01134-2, Mouse CD23 Recombinant Matched Antibody Pair - PBS only. 84212-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1295. 84212-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP01134-2

  Serum of six mouse was measured. The CD23 concentration of detected samples was determined to be 2624.00 pg/mL with a range of 1279.00 - 7445.00 pg/mL.

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  IHC staining of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84212-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84212-3-RR against Mouse CD23/Fc epsilon RII were performed. The affinity constant is 0.168 nM.

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Informations sur le produit

84212-3-PBS cible CD23 dans les applications de WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD23 Protéine recombinante Eg1295
• Nom complet: Fc receptor, IgE, low affinity II, alpha polypeptide
• Masse moléculaire calculée: 38kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: EDL21948.1
• Symbole du gène: Fcer2a
• Identification du gène (NCBI): 14128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD23/Fc ε RII) is a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21, involved in B cell differentiation and IgE regulation. Two forms of Fc ε RII (Fc ε RIIa and Fc ε RIIb) were found to exist, which differ in structure only in the N-terminal cytoplasmic region, and Fc ε RIIa and Fc ε RIIb play roles in effector cells of B-cell and IgE-mediated immunity, respectively. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, after IgE binding and antigen cross-linking, intracellular killing of parasites is induced by activation of the L-arginine-nitric oxide pathway.

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