Anticorps Monoclonal anti-Glycophorin A/CD235a

Glycophorin A/CD235a Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2b, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

HIR2

N° de cat : PE-65174

Synonymes

Glycophorin A, CD235a, GpMiIII, GPErik, GPA

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Galerie de données de validation

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  FC experiment of human red blood cells using PE-65174

  10 ul human whole blood was surface stained with 5 ul PE Anti-Human CD235a (HIR2) (PE-65174) or PE Mouse IgG2b Isotype Control. Cells were not fixed. Red blood cells were gated.

• 

  FC experiment of human red blood cells using PE-65174

  10 ul human whole blood was surface stained with 5 ul PE Anti-Human CD235a (HIR2) (PE-65174) or PE Mouse IgG2b Isotype Control. Cells were not fixed. Red blood cells were gated.

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Applications testées

• Résultats positifs en cytométrie: globules rouges humains

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65174 cible Glycophorin A/CD235a dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide synthétique (humain, N-terminal)
• Nom complet: glycophorin A (MNS blood group)
• Masse moléculaire calculée: 150 aa, 16 kDa
• Numéro d’acquisition GenBank: BC005319
• Symbole du gène: Glycophorin A
• Identification du gène (NCBI): 2993
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: L'anticorps purifié est conjugué à la R-phycoérithrine (PE) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

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