Anticorps Polyclonal de lapin anti-CD24

CD24 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10600-1-PBS

Synonymes

CD24A

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Galerie de données de validation

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  WB analysis using 10600-1-AP (same clone as 10600-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10600-1-AP (CD24 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10600-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 10600-1-AP (same clone as 10600-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10600-1-AP (CD24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10600-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 10600-1-AP (same clone as 10600-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 10600-1-AP (CD24 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10600-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 10600-1-AP (same clone as 10600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10600-1-AP (CD24 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10600-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 10600-1-AP (same clone as 10600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10600-1-AP (CD24 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10600-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10600-1-PBS cible CD24 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD24 Protéine recombinante Ag0654
• Nom complet: CD24 molecule
• Masse moléculaire calculée: 8 kDa
• Poids moléculaire observé: 30-70 kDa
• Numéro d’acquisition GenBank: BC007674
• Symbole du gène: CD24
• Identification du gène (NCBI): 100133941
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)

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