Anticorps Monoclonal anti-CD24

CD24 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

1H5C4

N° de cat : 67627-1-Ig

Synonymes

1H5C4, CD24A

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Galerie de données de validation

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  WB analysis using 67627-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67627-1-Ig (CD24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Daudi using 67627-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CD24 antibody (67627-1-Ig, Clone: 1H5C4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules Daudi, cellules HL-60, cellules Jurkat, cellules K-562, cellules Raji, cellules Ramos
• Résultats positifs en IF/ICC: cellules Daudi,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

67627-1-Ig cible CD24 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD24 Protéine recombinante Ag11679
• Nom complet: CD24 molecule
• Masse moléculaire calculée: 8 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC007674
• Symbole du gène: CD24
• Identification du gène (NCBI): 100133941
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)

Publications

Species	Application	Title
human	WB	Breast Cancer (Dove Med Press); CD24 May Serve as an Immunotherapy Target in Triple-Negative Breast Cancer by Regulating the Expression of PD-L1; Authors - Xudong Zhu
human	WB	Biomedicines; Development of LncRNA Biomarkers in Extracellular Vesicle of Amniotic Fluid Associated with Antenatal Hydronephrosis; Authors - Ying Fu
human	WB	Oncogene; A positive feedback loop of OTUD1 and c-Jun driven by leptin expedites stemness maintenance in ovarian cancer; Authors - Jingtao Wang

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