Anticorps Recombinant de lapin anti-CD244

CD244 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241260C6

N° de cat : 84086-1-PBS

Synonymes

SLAMF4, 241260C6, 2B4, h2B4, NAIL

---

Galerie de données de validation

• 

  WB analysis using 84086-1-RR (same clone as 84086-1-PBS)

  NK-92 cells were subjected to SDS PAGE followed by western blot with 84086-1-RR (CD244 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84086-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84086-1-RR (same clone as 84086-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84086-1-RR (CD244 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84086-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84086-1-RR (same clone as 84086-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84086-1-RR (CD244 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84086-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01008-3

  Cytometric bead array standard curve of MP01008-3, CD244 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84086-2-PBS. Detection antibody: 84086-1-PBS. Standard: Eg32087. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 84086-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84086-1-RR against Human CD244 were performed. The affinity constant is below 1 pM.

---

Informations sur le produit

84086-1-PBS cible CD244 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD244 Protéine recombinante Eg32087
• Nom complet: CD244 molecule, natural killer cell receptor 2B4
• Masse moléculaire calculée: 365 aa, 41 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC053985
• Symbole du gène: CD244
• Identification du gène (NCBI): 51744
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).

{{ptg:RelatedPrimaryAntibodies}}