Anticorps Polyclonal de lapin anti-CD247

CD247 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 12837-2-AP

Synonymes

CD247, CD247 molecule, CD3 ZETA, CD3H, CD3Q, CD3Z, T cell receptor T3 zeta chain, T3Z, TCRZ

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Galerie de données de validation

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  WB analysis of Jurkat using 12837-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12837-2-AP (CD247 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 12837-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12837-2-AP (CD247 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 12837-2-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12837-2-AP (CD247 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 12837-2-AP

  IP Result of anti-CD247 (IP:12837-2-AP, 3ug; Detection:12837-2-AP 1:700) with Jurkat cells lysate 1200ug.

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  IHC staining of human tonsillitis using 12837-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12837-2-AP (CD247 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12837-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12837-2-AP (CD247 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Jurkat using 12837-2-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using 12837-2-AP (CD247 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 12837-2-AP

  1X10^6 Jurkat cells were stained with 0.5ug CD247 antibody (12837-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu splénique de souris, tissu splénique humain
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules Jurkat,
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

12837-2-AP cible CD247 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD247 Protéine recombinante Ag3889
• Nom complet: CD247 molecule
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC025703
• Symbole du gène: CD247
• Identification du gène (NCBI): 919
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD247, also known as CD3Z (T-cell surface glycoprotein CD3 zeta chain), belongs to the CD3Z/FCER1G family. CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Publications

Species	Application	Title
human	WB	Front Immunol; The Transferrin Receptor-Directed CAR for the Therapy of Hematologic Malignancies.; Authors - Zilong Guo
mouse	WB	PLoS One; Liver Sinusoidal Endothelial Cell-Mediated CD8 T Cell Priming Depends on Co-Inhibitory Signal Integration over Time.; Authors - Julita Kaczmarek
human	IHC	Front Immunol; Distinct immune and inflammatory response patterns contribute to the identification of poor prognosis and advanced clinical characters in bladder cancer patients; Authors - Zhenglin Chang
human	FC	Cytotherapy; Bruton tyrosine kinase inhibitors preserve anti-CD19 chimeric antigen receptor T-cell functionality and reprogram tumor micro-environment in B-cell lymphoma; Authors - Wenjing Luo
human	WB,IP	Cell Rep; Targeting protein tyrosine phosphatases for CDK6-induced immunotherapy resistance; Authors - Xueliang Gao
mouse	WB	Nat Commun; Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function; Authors - Yuman Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Haizhen (Verified Customer) (10-15-2019)

specific for human CD3 zeta protein

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000 for western blot
• Cell Tissue Type: leukemia cell line

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