Anticorps Monoclonal anti-CD25/IL-2RA

CD25/IL-2RA Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

Publications(2)

CloneNo.

BC96

N° de cat : 65096-1-Ig

Synonymes

CD25, BC96, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha

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Galerie de données de validation

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  FC experiment of human PBMCs using 65096-1-Ig

  1x10^6 human PBMCs were stimulated with Human CD3/CD28 T cell Activation Beads Kit (KMS310) for 2 days then surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone: BC96) (right) or Mouse IgG1 Isotype Control (left). 1x10^6 unstimulated human PBMCs were surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone:BC96) (center). All samples were then stained with CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000 followed by CL488-conjugated Anti-Human CD4. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65096-1-Ig

  1x10^6 human PBMCs were stimulated with Human CD3/CD28 T cell Activation Beads Kit (KMS310) for 2 days then surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone: BC96) (red) or Mouse IgG1 Isotype Control (blue). 1x10^6 unstimulated human PBMCs were surface stained with 0.2 ug Anti-Human CD25 (65096-1-Ig, Clone:BC96) (black dashed). All samples were then stained with CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

65096-1-Ig cible CD25/IL-2RA dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: interleukin 2 receptor, alpha
• Masse moléculaire calculée: 272 aa, 31 kDa
• Numéro d’acquisition GenBank: BC156627
• Symbole du gène: IL2RA
• Identification du gène (NCBI): 3559
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

Publications

Species	Application	Title
human	FC	DNA Cell Biol; Upregulated Expression of microRNA-16 Correlates with Th17/Treg Cell Imbalance in Patients with Rheumatoid Arthritis.; Authors - Yuan-Hao Wu
		Allergy; Exploring CD26-/lo subpopulations of lymphocytes in asthma phenotype and severity: A novel CD4+ T cell subset expressing archetypical granulocyte proteins; Authors - Sara Vázquez-Mera

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