Anticorps Monoclonal anti-CD25/IL-2RA

CD25/IL-2RA Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG1, lambda

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

PC61.5

N° de cat : CL647-65137

Synonymes

CD25, Il-2ra, Il2ra, IL-2R subunit alpha, Il2r

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL647-65137

  1X10^6 mouse splenocytes were surface stained with CoraLite®488 Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD25 (CL647-65137, Clone: PC61.5) or CoraLite® Plus 647-Conjugated rat IgG2a isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65137 cible CD25/IL-2RA dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG1, lambda
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: interleukin 2 receptor, alpha chain
• Numéro d’acquisition GenBank: BC114437
• Symbole du gène: CD25
• Identification du gène (NCBI): 16184
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).

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