Anticorps Recombinant de lapin anti-CD25/IL-2R alpha

CD25/IL-2R alpha Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250670H3

N° de cat : 86213-1-PBS

Synonymes

CD25, IL-2 receptor subunit alpha, IL-2R subunit alpha, Il2ra, IL-2-RA

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Galerie de données de validation

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  WB analysis using 86213-1-RR (same clone as 86213-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 86213-1-RR (CD25/IL-2R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86213-1-PBS in a different storage buffer formulation.

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  WB analysis using 86213-1-RR (same clone as 86213-1-PBS)

  CTLL-2 cells were subjected to SDS PAGE followed by western blot with 86213-1-RR (CD25/IL-2R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86213-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02447-1

  Sandwich ELISA standard curve of MP02447-1, Rat CD25/IL-2RA Recombinant Matched Antibody Pair - PBS only. 86213-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1453. 86213-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP02447-1

  Serum of sixteen rats was measured. The CD25/IL-2RA concentration of detected samples was determined to be 0.89 ng/mL with a range of 0.44-5.27 ng/mL.

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Informations sur le produit

86213-1-PBS cible CD25/IL-2R alpha dans les applications de WB, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD25/IL-2R alpha Protéine recombinante Eg1453
• Nom complet: interleukin 2 receptor, alpha
• Masse moléculaire calculée: 31kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: NM_013163.1
• Symbole du gène: Il2ra
• Identification du gène (NCBI): 25704
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+ Foxp3+ Treg cells (PMID: 22585674).

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