Anticorps Recombinant de lapin anti-CD25/IL-2R alpha

CD25/IL-2R alpha Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250670H3

N° de cat : 86213-1-RR

Synonymes

CD25, IL-2 receptor subunit alpha, IL-2R subunit alpha, Il2ra, IL-2-RA

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Galerie de données de validation

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  WB analysis using 86213-1-RR

  Recombinant protein were subjected to SDS PAGE followed by western blot with 86213-1-RR (CD25/IL-2R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86213-1-RR

  CTLL-2 cells were subjected to SDS PAGE followed by western blot with 86213-1-RR (CD25/IL-2R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: CTLL-2 cells, Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86213-1-RR cible CD25/IL-2R alpha dans les applications de WB, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD25/IL-2R alpha Protéine recombinante Eg1453
• Nom complet: interleukin 2 receptor, alpha
• Masse moléculaire calculée: 31kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: NM_013163.1
• Symbole du gène: Il2ra
• Identification du gène (NCBI): 25704
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+ Foxp3+ Treg cells (PMID: 22585674).

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