Anticorps Recombinant de lapin anti-CD25/IL-2RA

CD25/IL-2RA Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242494H5

N° de cat : 98288-1-PBS

Synonymes

CD25, 242494H5, IL-2 receptor subunit alpha, Il2r, IL-2R subunit alpha

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98288-1-RR (same clone as 98288-1-PBS)

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD25/IL-2RA Rabbit RecAb (98288-1-RR, Clone: 242494H5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 750 Anti-Mouse CD4 (GK1.5) (CL750-65104, Clone: GK1.5). Cells were not fixed. This data was developed using the same antibody clone with 98288-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98288-1-RR (same clone as 98288-1-PBS)

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD25/IL-2RA Rabbit RecAb (98288-1-RR, Clone: 242494H5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD25/IL-2RA Rabbit RecAb (98288-1-RR, Clone: 242494H5) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98288-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98288-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98288-1-RR against Mouse CD25/IL-2RA were performed. The affinity constant is below 1 pM.

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Informations sur le produit

98288-1-PBS cible CD25/IL-2RA dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD25/IL-2RA Protéine recombinante Eg3087
• Nom complet: interleukin 2 receptor, alpha chain
• Masse moléculaire calculée: 31 kDa
• Numéro d’acquisition GenBank: NM_008367.3
• Symbole du gène: CD25
• Identification du gène (NCBI): 16184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

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