Anticorps Recombinant de lapin anti-CD25/IL-2RA

CD25/IL-2RA Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240906E11

N° de cat : 98469-1-RR

Synonymes

CD25, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA

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Galerie de données de validation

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  FC experiment of human PBMCs using 98469-1-RR

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD25/IL-2RA Rabbit RecAb (98469-1-RR, Clone: 240906E11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98469-1-RR

  1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD25/IL-2RA Rabbit RecAb (98469-1-RR, Clone: 240906E11) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD25/IL-2RA Rabbit RecAb (98469-1-RR, Clone: 240906E11) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98469-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98469-1-RR against Human CD25/IL-2RA were performed. The affinity constant is 0.387 nM.

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Applications testées

• Résultats positifs en cytométrie: PHA-treated human PBMCs

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98469-1-RR cible CD25/IL-2RA dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD25/IL-2RA Protéine recombinante Eg0408
• Nom complet: interleukin 2 receptor, alpha
• Masse moléculaire calculée: 272 aa, 31 kDa
• Numéro d’acquisition GenBank: BC156627
• Symbole du gène: IL2RA
• Identification du gène (NCBI): 3559
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

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