Anticorps Recombinant de lapin anti-CD25/IL-2RA

CD25/IL-2RA Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, primates non humains, singe

Applications

FC

Conjugaison

Biotin

CloneNo.

BC96

N° de cat : Biotin-65620

Synonymes

CD25, BC96, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha

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Galerie de données de validation

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  FC experiment of human PBMCs using Biotin-65620

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD25/IL-2RA (BC96) Mouse IgG2a RecAb (Biotin-65620, Clone: BC96) and CoraLite®488-conjugated streptavidin. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD4 Rabbit Recombinant Antibody (CL647-98042, Clone: 240427E12). Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-65620

  1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD25/IL-2RA (BC96) Mouse IgG2a RecAb (Biotin-65620, Clone: BC96) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD25/IL-2RA (BC96) Mouse IgG2a RecAb (Biotin-65620, Clone: BC96) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: PHA treated human PBMCs

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-65620 cible CD25/IL-2RA dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains, singe

• Réactivité: Humain, primates non humains, singe
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: interleukin 2 receptor, alpha
• Masse moléculaire calculée: 272 aa, 31 kDa
• Numéro d’acquisition GenBank: BC156627
• Symbole du gène: IL2RA
• Identification du gène (NCBI): 3559
• Conjugaison: Biotin
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

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