Anticorps Recombinant de lapin anti-CD25/IL-2RA

CD25/IL-2RA Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

242494H5

N° de cat : PE-FcA98288

Synonymes

CD25, IL-2 receptor subunit alpha, Il2r, IL-2R subunit alpha, Il2ra

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Galerie de données de validation

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  FC experiment of mouse splenocytes using PE-FcA98288

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.1 ug PE Anti-Mouse CD25/IL-2RA Rabbit RecAb (PE-FcA98288, Clone: 242494H5) and FcZero-rAb™ APC Anti-Mouse CD4 (RM4-4). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Anti-CD3/CD28 treated mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-FcA98288 cible CD25/IL-2RA dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD25/IL-2RA Protéine recombinante Eg3087
• Nom complet: interleukin 2 receptor, alpha chain
• Masse moléculaire calculée: 31 kDa
• Numéro d’acquisition GenBank: NM_008367.3
• Symbole du gène: CD25
• Identification du gène (NCBI): 16184
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

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