Anticorps Recombinant de lapin anti-CD27

CD27 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

O323

N° de cat : 65627-1-PBS

Synonymes

CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27

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Galerie de données de validation

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  FC experiment of human PBMCs using 65627-1-MR (same clone as 65627-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD27 (O323) Mouse IgG2a Recombinant Antibody (65627-1-MR, Clone:O323) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65627-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65627-1-MR (same clone as 65627-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD27 (O323) Mouse IgG2a Recombinant Antibody (65627-1-MR, Clone: O323) (red) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65627-1-PBS in a different storage buffer formulation.

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Informations sur le produit

65627-1-PBS cible CD27 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD27 molecule
• Masse moléculaire calculée: 29 kDa
• Numéro d’acquisition GenBank: BC012160
• Symbole du gène: CD27
• Identification du gène (NCBI): 939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

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