Anticorps Recombinant de lapin anti-CD27

CD27 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241378E11

N° de cat : 98160-1-PBS

Synonymes

Tumor necrosis factor receptor superfamily member 7, Tnfrsf7, T-cell activation antigen CD27, CD27L receptor, CD27 antigen

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98160-1-RR (same clone as 98160-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD27 Rabbit Recombinant Antibody (98160-1-RR, Clone:241378E11) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). cells were co-stained with APC Anti-mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 98160-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98160-1-RR (same clone as 98160-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD27 Rabbit Recombinant Antibody (98160-1-RR, Clone: 241378E11) (red), or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98160-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98160-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98160-1-RR against Mouse CD27 were performed. The affinity constant is 1.87 nM.

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Informations sur le produit

98160-1-PBS cible CD27 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD27 antigen
• Masse moléculaire calculée: 28kDa
• Numéro d’acquisition GenBank: NM_001033126.2
• Symbole du gène: CD27
• Identification du gène (NCBI): 21940
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

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