Anticorps Monoclonal anti-CD27

CD27 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

O323

N° de cat : CL488-65132

Synonymes

CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55

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Galerie de données de validation

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  FC experiment of human peripheral blood lymphocytes using CL488-65132

  1X10^6 human peripheral blood lymphocytes were surface stained with APC-Anti-Human CD19 (APC-65145, Clone: SJ25C1) and 5.00 ul CoraLite® Plus 488-conjugated Anti-Human CD27 (CL488-65132, Clone: O323). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using CL488-65132

  1X10^6 human peripheral blood lymphocytes were surface stained with APC-Anti-Human CD19 (APC-65145, Clone: SJ25C1) and CoraLite® Plus 488-conjugated Mouse IgG1 isotype control antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65132 cible CD27 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD27 molecule
• Masse moléculaire calculée: 29 kDa
• Numéro d’acquisition GenBank: BC012160
• Symbole du gène: CD27
• Identification du gène (NCBI): 939
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à une température comprise entre 2 °C et 8 °C. Éviter toute exposition à la lumière.

Informations générales

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.