Anticorps Recombinant de lapin anti-CD27

CD27 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

241378E11

N° de cat : PE-98160

Synonymes

Tumor necrosis factor receptor superfamily member 7, Tnfrsf7, T-cell activation antigen CD27, CD27L receptor, CD27 antigen

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Galerie de données de validation

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  FC experiment of mouse splenocytes using PE-98160

  1x10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2), and 0.25 ug PE Anti-Mouse CD27 Rabbit RecAb (PE-98160, Clone:241378E11) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9). Cells were not fixed.

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  FC experiment of mouse splenocytes using PE-98160

  1x10^6 mouse splenocytes were surface stained with 0.25 ug PE Anti-Mouse CD27 Rabbit RecAb (PE-98160, Clone:241378E11) (red) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-98160 cible CD27 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD27 antigen
• Masse moléculaire calculée: 28kDa
• Numéro d’acquisition GenBank: NM_001033126.2
• Symbole du gène: CD27
• Identification du gène (NCBI): 21940
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

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