Anticorps Recombinant de lapin anti-CD27

CD27 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

PE-Cyanine7 Fluorescent Dye

CloneNo.

242731G8

N° de cat : PY7-98346-3

Synonymes

242731G8, CD27 antigen, CD27 molecule, CD27L receptor, S152

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Galerie de données de validation

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  FC experiment of human PBMCs using PY7-98346-3

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3, and 5 ul PE-Cyanine7 Anti-Human CD27 Rabbit RecAb (PY7-98346-3, Clone: 242731G8) or unstained. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using PY7-98346-3

  1x10^6 human PBMCs were surface stained with 5 ul PE-Cyanine7 Anti-Human CD27 Rabbit RecAb (PY7-98346-3, Clone:242731G8) (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PY7-98346-3 cible CD27 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD27 Protéine recombinante Eg1644
• Nom complet: CD27 molecule
• Masse moléculaire calculée: 29 kDa
• Numéro d’acquisition GenBank: BC012160
• Symbole du gène: CD27
• Identification du gène (NCBI): 939
• Conjugaison: PE-Cyanine7 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 450-500 nm / 778 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

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