Anticorps Monoclonal anti-ICOSLG/B7-H2/CD275

ICOSLG/B7-H2/CD275 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

HK5.3

N° de cat : PE-65114

Synonymes

CD275, B7-H2, Icosl, icos ligand, ICOS L

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Galerie de données de validation

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  FC experiment of mouse splenocytes using PE-65114

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse CD3 (APC-65077, Clone: 17A2) and 0.06 ug PE Anti-Mouse CD275 (B7-H2) (PE-65114, Clone: HK5.3). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PE-65114

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse CD3 (APC-65077, Clone: 17A2) and 0.06 ug PE-Rat IgG2a isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65114 cible ICOSLG/B7-H2/CD275 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée cellulaire transfectée par B7-RP1 de souris
• Nom complet: icos ligand
• Numéro d’acquisition GenBank: BC029227
• Symbole du gène: CD275
• Identification du gène (NCBI): 50723
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.1% gelatin
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD275, also known as B7-H2, ICOSLG or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. CD275 is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of CD275/ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).

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