Anticorps Monoclonal anti-PD-1/CD279

PD-1/CD279 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain, primates non humains

Applications

FC

Conjugaison

Non conjugué

CloneNo.

EH12.2H7

N° de cat : 65168-1-Ig

Synonymes

CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

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Galerie de données de validation

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  FC experiment of human PBMCs using 65168-1-Ig

  1X10^6 day 3 PHA treated human PBMCs were surface stained with 0.2 ug Anti-Human PD-1/CD279 (65168-1-Ig, Clone:EH12.2H7) and CL594-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. Blue line indicates unstained stimulated cells. Black dashed line indicates unstimulated stained cells.

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Applications testées

• Résultats positifs en cytométrie: PHA treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65168-1-Ig cible PD-1/CD279 dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains

• Réactivité: Humain, primates non humains
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Recombinant human CD279 protein
• Nom complet: programmed cell death 1
• Masse moléculaire calculée: 288 aa, 32 kDa
• Numéro d’acquisition GenBank: BC074740
• Symbole du gène: PD-1
• Identification du gène (NCBI): 5133
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par chromatographie d'affinité par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

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