Anticorps Recombinant de lapin anti-CD28

CD28 Recombinant Antibody for IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240354D2

N° de cat : 83383-6-RR

Synonymes

CD28, CD28 molecule, Tp44

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Galerie de données de validation

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  IF Staining of Jurkat using 83383-6-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells and THP-1 cells (negative control) using CD28 antibody (83383-6-RR, Clone: 240354D2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  IF Staining of Jurkat using 83383-6-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells and THP-1 cells (negative control) using CD28 antibody (83383-6-RR, Clone: 240354D2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  Affinity and Kinetic Characterization of 83383-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83383-6-RR against Human CD28 were performed. The affinity constant is 1.99 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules Jurkat,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83383-6-RR cible CD28 dans les applications de IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD28 molecule
• Masse moléculaire calculée: 220 aa, 25 kDa
• Numéro d’acquisition GenBank: BC093698
• Symbole du gène: CD28
• Identification du gène (NCBI): 940
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).

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