Anticorps Monoclonal anti-CD3

• Phare
• Validé par KD/KO

CD3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3F3A1

N° de cat : 60181-1-PBS

Synonymes

CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon

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Galerie de données de validation

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  WB analysis using 60181-1-Ig (same clone as 60181-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60181-1-Ig (CD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60181-1-Ig (same clone as 60181-1-PBS)

  WB result of CD3 antibody (60181-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD3 transfected Jurkat cells. This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig (CD3 antibody) at dilution of 1:6400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60181-1-Ig (same clone as 60181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60181-1-Ig (same clone as 60181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD20 antibody (24828-1-AP, green). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD20 antibody (24828-1-AP, green). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3), FOXP3 antibody (22228-1-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60181-1-Ig (CD3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60181-1-Ig (same clone as 60181-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD20 antibody (24828-1-AP, green). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 60181-1-Ig (same clone as 60181-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60181-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50222-1

  Cytometric bead array standard curve of MP50222-1, CD3E Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60181-2-PBS. Detection antibody: 60181-1-PBS. Standard:Ag11797. Range: 0.098-50 ng/mL.

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Informations sur le produit

60181-1-PBS cible CD3 dans les applications de WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD3 Protéine recombinante Ag11797
• Nom complet: CD3e molecule, epsilon (CD3-TCR complex)
• Masse moléculaire calculée: 207 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC049847
• Symbole du gène: CD3
• Identification du gène (NCBI): 916
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.

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