Anticorps Monoclonal anti-CD3

CD3 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

Humain

Applications

IHC, FC, Cell treatment

Conjugaison

Non conjugué

Publications(3)

CloneNo.

OKT3

N° de cat : 65133-1-Ig

Synonymes

CD3E, CD3 epsilon, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, OKT3

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Galerie de données de validation

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  FC experiment of human PBMCs using 65133-1-Ig

  1X10^6 human PBMCs were surface co-stained with FITC Anti-human CD19 and 0.2 ug Anti-Human CD3 (65133-1-Ig, Clone: OKT3) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG2a (KFA043) or CoraLite® Plus 647 conjugated Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65133-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 (65133-1-Ig, Clone: OKT3) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG2a (KFA043) (red) or CoraLite® Plus 647 conjugated Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

65133-1-Ig cible CD3 dans les applications de IHC, FC, Cell treatment et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Human T cells
• Nom complet: CD3e molecule, epsilon (CD3-TCR complex)
• Masse moléculaire calculée: 207 aa, 23 kDa
• Numéro d’acquisition GenBank: BC049847
• Symbole du gène: CD3
• Identification du gène (NCBI): 916
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

Species	Application	Title
mouse	IHC	Front Immunol; Efficacy and safety evaluation of cross-reactive Fibroblast activation protein scFv-based CAR-T cells; Authors - Wenhao Niu
human	Cell treatment	J Pharm Anal; Secondary metabolites of mulberry leaves exert anti-lung cancer activity through regulating the PD-L1/PD-1 signaling pathway; Authors - Guiqin Ye
		Signal Transduct Target Ther; N-deglycosylation targeting chimera (DGlyTAC): a strategy for immune checkpoint proteins inactivation by specifically removing N-glycan; Authors - Li Li

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