Anticorps Monoclonal anti-CD3

CD3 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

Publications(1)

CloneNo.

SK7

N° de cat : 65148-1-Ig

Synonymes

CD3E, CD3 epsilon, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, SK7

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Galerie de données de validation

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  FC experiment of human PBMCs using 65148-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 (65148-1-Ig, Clone:SK7) (red) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) (blue) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65148-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 (65148-1-Ig, Clone:SK7) (red) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) (blue) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

65148-1-Ig cible CD3 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD3e molecule, epsilon (CD3-TCR complex)
• Masse moléculaire calculée: 207 aa, 23 kDa
• Numéro d’acquisition GenBank: BC049847
• Symbole du gène: CD3
• Identification du gène (NCBI): 916
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

Species	Application	Title
human	FC	Biochem Biophys Rep; Evaluation of an anti-CD3 VHH and construction of an anti-CD3/anti-EGFR bispecific tandem VHH as a cancer cell targeting drug construct; Authors - Takuya Asanuma

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