Anticorps Recombinant de lapin anti-CD3

CD3 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242012G2

N° de cat : 84505-4-PBS

Synonymes

CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon

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Galerie de données de validation

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  WB analysis using 84505-4-RR (same clone as 84505-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84505-4-RR (CD3E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84505-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84505-4-RR (same clone as 84505-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84505-4-RR (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84505-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84505-4-RR (same clone as 84505-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84505-4-RR (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84505-4-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 84505-4-RR (same clone as 84505-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD3 antibody (84505-4-RR, Clone: 242012G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84505-4-PBS in a different storage buffer formulation.

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Informations sur le produit

84505-4-PBS cible CD3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD3e molecule, epsilon (CD3-TCR complex)
• Masse moléculaire calculée: 207 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC049847
• Symbole du gène: CD3
• Identification du gène (NCBI): 916
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This antibody is directed against the epsilon chain of human CD3 molecule.

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