Anticorps Recombinant de lapin anti-CD3

CD3 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

APC Fluorescent Dye

Publications(1)

CloneNo.

UCHT1

N° de cat : APC-65570

Synonymes

CD3E, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T3E

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Galerie de données de validation

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  FC experiment of human PBMCs using APC-65570

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD19 and 5 ul APC Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (APC-65570, Clone: UCHT1) (right), or APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4) (left). Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using APC-65570

  1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (APC-65570, Clone: UCHT1) (red), or APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65570 cible CD3 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
• Nom complet: CD3e molecule, epsilon (CD3-TCR complex)
• Masse moléculaire calculée: 207 aa, 23 kDa
• Numéro d’acquisition GenBank: BC049847
• Symbole du gène: CD3
• Identification du gène (NCBI): 916
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

Species	Application	Title
		Anal Chem; A High-Efficiency Electrochemical Biosensor for the Detection of Mucosal-Associated Invariant T Cells; Authors - Zhenguo Zhang

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