Anticorps Recombinant de lapin anti-CD3
CD3 Recombinant Antibody for FC
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain
Applications
FC
Conjugaison
APC Fluorescent Dye
CloneNo.
UCHT1
N° de cat : APC-FcA65570
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en cytométrie | CMSP humaines, |
Dilution recommandée
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
APC-FcA65570 cible CD3 dans les applications de FC et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Recombinant |
| Type | Anticorps |
| Immunogène | Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor |
| Nom complet | CD3e molecule, epsilon (CD3-TCR complex) |
| Masse moléculaire calculée | 207 aa, 23 kDa |
| Numéro d’acquisition GenBank | BC049847 |
| Symbole du gène | CD3 |
| Identification du gène (NCBI) | 916 |
| Conjugaison | APC Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 650 nm / 660 nm |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS with 0.09% sodium azide and 0.5% BSA |
| Conditions de stockage | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Informations générales
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
Protocole
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD3 antibody APC-FcA65570 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Pedro (Verified Customer) (06-12-2025) | When I tested the FcZero-rAb APC Anti-Human CD3 (UCHT1) Rabbit IgG Recombinant antibody on PBMCs, I confirmed that the staining was very visible, with the cell population well discriminated. It's an advantage that, with this antibody, I don't need to use FcR Block reagent, and I can't still visualize such an outstanding staining. I approve and recommend the product.
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