Anticorps Recombinant de lapin anti-CD3

CD3 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Lapin

Applications

FC

Conjugaison

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

242568D11

N° de cat : CL405-98349

Synonymes

CD3 epsilon + CD3 gamma, CD3 epsilon/CD3 gamma, CD3 epsilon, CD3 gamma, CD3e

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Galerie de données de validation

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  FC experiment of rabbit splenocytes using CL405-98349

  1x10^6 rabbit splenocytes were surface stained with FITC Anti-Rabbit CD4, and 0.25 ug CoraLite® Plus 405 Anti-Rabbit CD3 Mouse RecAb (CL405-98349, Clone: 242568D11) or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4). Cells were not fixed.

• 

  FC experiment of rabbit splenocytes using CL405-98349

  1x10^6 rabbit splenocytes were surface stained with 0.25 ug CoraLite® Plus 405 Anti-Rabbit CD3 Mouse RecAb (CL405-98349, Clone: 242568D11) (red) or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: rabbit splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL405-98349 cible CD3 dans les applications de FC et montre une réactivité avec des échantillons Lapin

• Réactivité: Lapin
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD3 Protéine recombinante Eg3525
• Nom complet: T-cell surface glycoprotein CD3 epsilon chain
• Symbole du gène: CD3E
• Identification du gène (NCBI): 100008616
• Conjugaison: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 399 nm / 422 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD3 epsilon(CD3ε) and CD3 gamma (CD3γ) are transmembrane subunits of the CD3 complex, which is essential for the assembly and surface expression of the T cell receptor (TCR). All of the CD3 subunits carry the so-called immunoreceptor tyrosine-based activation motif (ITAM) in the intracytoplasmic region. The signals are initiated by the specific phosphorylation of two tyrosine residues located in the conserved ITAM (PMID: 21269565).

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