Anticorps Monoclonal anti-CD3

CD3 Monoclonal Antibody for IF/ICC, FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

IF/ICC, FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(1)

CloneNo.

17A2

N° de cat : CL488-65077

Synonymes

AI504783, CD3, Cd3e, CD3epsilon, T3e

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Galerie de données de validation

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  IF Staining of mouse splenocytes using CL488-65077

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD3 (CL488-65077, Clone: 17A2) at dilution of 1:500.

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  FC experiment of mouse splenocytes using CL488-65077

  1X10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD3 (CL488-65077, Clone:17A2) or 0.5 ug CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2), and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were not fixed.

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Applications testées

• Résultats positifs en IF/ICC: splénocytes de souris,
• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

CL488-65077 cible CD3 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Hybridome T-T γδTCR-positif D1
• Nom complet: CD3 antigen, epsilon polypeptide
• Numéro d’acquisition GenBank: BC098236
• Symbole du gène: CD3ε
• Identification du gène (NCBI): 12501
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

Species	Application	Title
mouse	FC	Int J Biol Macromol; Polysaccharide-mediated combination therapy enhances anti-tumor effects by promoting the immune cycle of immunogenic cell death; Authors - Xiang-Long Yu

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