Anticorps Monoclonal anti-CD3

CD3 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

Hit3a

N° de cat : CL555-65112

Synonymes

CD3E, T3E, TCRE



Applications testées

Résultats positifs en cytométrieCMSP humaines,

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-65112 cible CD3 dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG2a, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène s/o
Nom complet CD3e molecule, epsilon (CD3-TCR complex)
Masse moléculaire calculée 207 aa, 23 kDa
Numéro d’acquisition GenBankBC049847
Symbole du gène CD3E
Identification du gène (NCBI) 916
Conjugaison CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS with 0.09% sodium azide and 0.5% BSA.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocole

Product Specific Protocols
FC protocol for CL Plus 555 CD3 antibody CL555-65112Download protocol
Standard Protocols
Click here to view our Standard Protocols