Anticorps Monoclonal anti-CD3

CD3 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

Humain

Applications

IF, FC

Conjugaison

FITC Plus Fluorescent Dye

Publications(4)

CloneNo.

OKT3

N° de cat : FITC-65133

Synonymes

CD3E, CD3 epsilon, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, OKT3

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Galerie de données de validation

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  FC experiment of human PBMCs using FITC-65133

  1x10^6 human PBMCs were surface stained with CL405 Anti-Human CD19 (CL405-65197) and 5ul FITC Plus Anti-Human CD3 (FITC-65133, Clone:OKT3) or FITC Plus Mouse IgG2a Isotype Control (FITC-65244, Clone: MOPC-173). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65133

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD3 (FITC-65133, Clone:OKT3) or FITC Plus Mouse IgG2a Isotype Control (FITC-65244, Clone: MOPC-173). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below
• FC: See 2 publications below

Informations sur le produit

FITC-65133 cible CD3 dans les applications de IF, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Human T cells
• Nom complet: CD3e molecule, epsilon (CD3-TCR complex)
• Masse moléculaire calculée: 207 aa, 23 kDa
• Numéro d’acquisition GenBank: BC049847
• Symbole du gène: CD3
• Identification du gène (NCBI): 916
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

Species	Application	Title
		Int J Pharm; A self-assembly active nanomodulator based on berberine for photothermal immunotherapy of breast cancer via dual regulation of immune suppression; Authors - Jiang Sun
human	FC	J Biol Eng; Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units; Authors - Jyunna Yoshida
	IF	J Med Chem; Platinum(IV) Complexes as Inhibitors of STAT3 and Regulators of the Tumor Microenvironment To Control Breast Cancer; Authors - Linxiang Cai
human	FC	Sci Rep; Construction of IgG-Fab2 bispecific antibody via intein-mediated protein trans-splicing reaction; Authors - Risa Yamada

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