Anticorps Monoclonal anti-CD3 Delta

CD3 Delta Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A12H2

N° de cat : 60194-1-PBS

Synonymes

CD3D, 3A12H2, CD3-delta, T cell receptor T3 delta chain, T3D

---

Galerie de données de validation

• 

  WB analysis of jurkat using 60194-1-Ig (same clone as 60194-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60194-1-Ig (CD3 delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60194-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50691-2

  Cytometric bead array standard curve of MP50691-2, CD3D/CD3 Delta Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60194-1-PBS. Detection antibody: 60194-4-PBS. Standard:Ag10207. Range: 0.781-100 ng/mL.

---

Informations sur le produit

60194-1-PBS cible CD3 Delta dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD3 Delta Protéine recombinante Ag10207
• Nom complet: CD3d molecule, delta (CD3-TCR complex)
• Masse moléculaire calculée: 171 aa, 19 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC070321
• Symbole du gène: CD3 Delta
• Identification du gène (NCBI): 915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. CD3 delta/CD3D is a single-pass type I membrane protein which consists of an extracellular domain of 84 amino acids, a transmembrane region, and a cytoplasmic domain of 45 amino acids. Defects in CD3 delta are a cause of severe combined immunodeficiency.

{{ptg:RelatedPrimaryAntibodies}}