Anticorps Recombinant de lapin anti-CD3 epsilon

CD3 epsilon Recombinant Antibody for WB, IF-P, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IF-P, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242849D3

N° de cat : 86053-1-PBS

Synonymes

CD3e, T-cell surface glycoprotein CD3 epsilon chain

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Galerie de données de validation

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  WB analysis using 86053-1-RR (same clone as 86053-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86053-1-RR (CD3 epsilon/CD3e antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

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  WB analysis of CTLL-2 using 86053-1-RR (same clone as 86053-1-PBS)

  CTLL-2 cells were subjected to SDS PAGE followed by western blot with 86053-1-RR (CD3 epsilon/CD3e antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02251-2

  The mean CD3 epsilon concentration was determined to be 6.3 ng/mL in rat heart tissue extract based on a 6.7 mg/mL extract load and 13.6 ng/mL in rat spleen tissue extract based on a 2.7 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02251-2

  Sandwich ELISA standard curve of MP02251-2, Rat CD3 epsilon Recombinant Matched Antibody Pair - PBS only. 86053-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1450. 86053-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  IF Staining of rat thymus using 86053-1-RR (same clone as 86053-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat thymus tissue using CD3 epsilon antibody (86053-1-RR, Clone: 242849D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

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  IF Staining of rat thymus using 86053-1-RR (same clone as 86053-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat thymus tissue using CD3 epsilon antibody (86053-1-RR, Clone: 242849D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat spleen using 86053-1-RR (same clone as 86053-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD3 epsilon antibody (86053-1-RR, Clone: 242849D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86053-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86053-1-PBS cible CD3 epsilon dans les applications de WB, IF-P, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD3 epsilon Protéine recombinante Eg1450
• Nom complet: CD3 molecule, epsilon polypeptide
• Masse moléculaire calculée: 22kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: NP_001101610.2
• Symbole du gène: Cd3e
• Identification du gène (NCBI): 315609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657). This antibody was raised against the epsilon chain of rat CD3 molecule.

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