Anticorps Recombinant de lapin anti-CD30 Ligand/TNFSF8

CD30 Ligand/TNFSF8 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241831D9

N° de cat : 98253-1-PBS

Synonymes

TNFSF 8, CD30 L, TNFSF8, 241831D9, CD30LG

---

Galerie de données de validation

• 

  FC experiment of human PBMCs using 98253-1-RR (same clone as 98253-1-PBS)

  1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. This data was developed using the same antibody clone with 98253-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98253-1-RR (same clone as 98253-1-PBS)

  1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98253-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98253-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98253-1-RR against Human CD30 Ligand/TNFSF8 were performed. The affinity constant is below 1 pM.

---

Informations sur le produit

98253-1-PBS cible CD30 Ligand/TNFSF8 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: tumor necrosis factor (ligand) superfamily, member 8
• Masse moléculaire calculée: 234 aa, 26 kDa
• Numéro d’acquisition GenBank: BC093630
• Symbole du gène: TNFSF8
• Identification du gène (NCBI): 944
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD30 Ligand (CD30L), also named as TNFSF8 or CD153, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) superfamily (PMID: 8391931). It is expressed on the surface of activated T cells, B cells, monocytes, macrophages, eosinophils, neutrophils, and mast cells (PMID: 26090498). CD30L interacts with its receptor, CD30, which is a cell surface protein expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin lymphoma and anaplastic large cell lymphomas (PMID: 28885612). The CD30/CD30L signaling is involved in pleiotropic downstream effects including differentiation, cell survival and death, NFkB activation, and production of cytokines (PMID: 38534788).

{{ptg:RelatedPrimaryAntibodies}}