Anticorps Recombinant de lapin anti-CD300e

CD300e Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242031C10

N° de cat : 98235-1-RR

Synonymes

CMRF35A5, CMRF35 A5, CLM2, CLM 2, CD300LE

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Galerie de données de validation

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  FC experiment of human PBMCs using 98235-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD300e Rabbit Recombinant Antibody (98235-1-RR, Clone: 242031C10) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed

• 

  FC experiment of human PBMCs using 98235-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD300e Rabbit Recombinant Antibody (98235-1-RR, Clone: 242031C10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98235-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98235-1-RR against Human CD300e were performed. The affinity constant is 0.456 nM.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98235-1-RR cible CD300e dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD300e molecule
• Masse moléculaire calculée: 23 kDa
• Numéro d’acquisition GenBank: NM_181449.2
• Symbole du gène: CD300E
• Identification du gène (NCBI): 342510
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD300e, also known as immune receptor expressed on myeloid cells 2 (IREM-2), CLM-2, or CMRF35-A5, is one of the CD300 family members which belong to the immunoglobulin (Ig) superfamily (PMID: 15557162; 20039296). CD300e is a type I transmembrane glycoprotein that contains an extracellular region comprising an Ig-like domain, a transmembrane region, and a short cytoplasmic tail (PMID: 15557162; 29358324). It is by monocytes and myeloid dendritic cells and transmits an immune-activating signal by interacting with DNAX-activating protein 12 (DAP12) (PMID: 29358324).

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