Anticorps Recombinant de lapin anti-CD302

CD302 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242893D7

N° de cat : 85813-5-PBS

Synonymes

CD302 antigen, Clec13a, C-type lectin domain family 13 member A, Type I transmembrane C-type lectin receptor DCL-1

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Galerie de données de validation

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  WB analysis of rat liver using 85813-5-RR (same clone as 85813-5-PBS)

  Rat liver tissue was subjected to SDS PAGE, followed by western blot with 85813-5-RR (Cd302 antibody) at a dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 85813-5-RR (same clone as 85813-5-PBS)

  Mouse liver tissue was subjected to SDS PAGE followed by western blot with 85813-5-RR (Cd302 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 85813-5-RR (same clone as 85813-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 85813-5-RR (same clone as 85813-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 85813-5-RR (same clone as 85813-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 85813-5-RR (same clone as 85813-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

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Informations sur le produit

85813-5-PBS cible CD302 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD302 Protéine recombinante Eg2660
• Nom complet: CD302 antigen
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: NM_025422.4
• Symbole du gène: Cd302
• Identification du gène (NCBI): 66205
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD302 is a C-type lectin receptor involved in cell adhesion and migration, as well as endocytosis and phagocytosis. In mice and humans, CD302 is primarily expressed in the liver, lungs, lymph nodes (LNs), spleen, and bone marrow. In CD302 gene knockout (CD302KO) mice, the migration frequency and number of myeloid dendritic cells (mDC) in CD302KO lymph nodes were reduced compared to the wild type, confirming the functional role of CD302 in mDC migration.

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